Reducing contamination in your incubator is a continuous challenge. One of the easiest things to start with is to choose cell culture equipment that guards against contamination – equipment that is focused on temperature, gas concentrations and humidity.

  • Install your incubator where there is minimal foot traffic and where air disturbance is reduced.
  • Avoid installing your incubator near window air conditioners, ceiling or floor HVAC air diffusers or return air intakes, all of which are sources of airborne contamination
  • The CO2 or other gas supply tubing should be fitted with a 0.3 micron filter prior to flow into the incubator solenoid controls. Using a long tubing section makes it easy to access the filter cartridge to replace the filter medium.
  • Use a 70% ethanol solution to wipe down the interior or your incubator. Intentional dilution is to give the ethanol time to kill the contaminant before the ethanol evaporates.
  • Make sure cell culture apparatus such as orbital or reciprocal shakers, rockers, cell bottle rollers, magnetic stirrers and other devices are free of contaminants before they are placed in the incubator.

 

Learn how to lessen contamination risks and help improve your reproducibility. Download our application note “Mitigating Contamination in the Cell Culture Incubator.”

Previously, Creighton researchers were carting their glassware to lower floors or even hand washing their glassware.  This ineffective time-wasting procedure has been rectified by the newly installed Lancer 1400 LXP unit. It has a larger and wider chamber for greater capacity and volume. Now multiple labs in the department are currently utilizing the washer which in turn has increased lab productivity.   The department has been very happy with the new washer and has taken full advantage of the additional time freed up during the day.  
The Lancer 1400 LXP washer has the capability of 3 levels of washing in one cycle and easily adjustable level positions of each rack.  This allows great flexibility for various glassware types and sizes, plus allows the capability of multiple sizes of glassware to be ran in one cycle.  Along with this flexibility the washer also provides chamber and injector drying capabilities.  

Direct injection cleaning, HEPA filtered injector drying

The 1400 LXP provides injection washing and drying on one to three levels simultaneously, delivering the wash/rinse solutions into narrow-necked glassware to provide for efficient and effective cleaning. 4 Washing positions are available and adjustable for the upper levels and provide for greater wash load flexibility. Low profile, rear manifold connections provide wash/rinse solution to the racks, maximizing usable rack surface, which is often reduced by common center manifold designs. 100% of the chamber volume is then available for an unbeaten washing capacity in this size/range of glassware washer dryer. HEPA filtered chamber and direct injection drying, adjustable by 1º C increments, reduces cycle time by drying glassware and labware inside and out. This fast turnaround of glassware increases productivity by reducing the waiting time for glassware.

 

Ease of Use

The 1400 LXP features microprocessor controls with 4 pre-set programs for chemistry glassware, bacteriology/virology (high temperature), stubborn stains (agar) and volumetric glassware (lower temperature).  For other applications, the 1400 LXP offers 36 additional programs that can be modified to fit any application. These custom programs offer the user the ability to easily adjust cycle parameters for program phases, temperatures, phase times and chemical dosing. The system control panel is user-friendly with a 7 button keypad and 2 ½ inch LCD interface. Self-diagnostic software provides visual and audible alarms to quickly alert the user in the event of a malfunction, displaying the error and allowing the owner to avoid costly service calls. Additionally, with on-board storage capacity, the 1400 LXP provides added safety.  

 

Unbeaten cleaning and drying results

Lancer’s superior hydraulic design maximizes flow and pressure with a single, direct fluid
path and no intermediate accessories. Additionally, racks are held in place by the door to ensure a positive connection without the loss of pressure and flow that is common with other designs. Top and bottom spray arms provide thorough coverage for the interior and exterior of glassware. Water rinses are programmable for hot, cold and hot/cold DI water. These features allow for maximum use of the space within the chamber and ensure cleanliness. This design enhances full interchangeability of the racks between levels, providing further flexibility and reducing the number or racks needed. 


Inventory systems

Having the right rack or accessory to fit your cleaning application is key to ensuring cleanliness and increasing productivity. With this in mind, Lancer provides versatile rack systems to accommodate all types of glassware and laboratory items. These rack systems include a wide range of accessories with various configurations of sizes and quantities of jets or baskets to handle all types of glass and labware from bottles to pipettes. Racks can be interchanged between levels to achieve further flexibility.

Lancer manufacturers a wide range of washers sizing from undercounters to large stand-alone units. Our DAI’s sales rep are thoroughly trained on Lancer’s full line of washers. They work with end-users to understand each facility’s washing needs and what product will work best. Contact us today to find out which one is best for your lab.
 

AALAS’s National Meeting is just around the corner, and we couldn’t be more excited. Your local DAI rep group will be at both the Consolidated Sterilizer Systems Booth (#545) and Lynx Booth (#433)

Consolidated will be showcasing its state-of-the-art X1TM sterilizer control system. Designed and programmed with “ease-of-use” in mind, the X1 Controller enables technicians to program up to 50 sterilization cycles and features the ability to create “favorite” cycle recipes.

Lynx will be showcasing their rack washers, cannel washer, bedding handle systems. The Lynx Mission to provide Animal Care washing systems of the highest standard, quality, and reliability in the industry.

Hosted by the American Association for Laboratory Animal Science, the National Meeting is the largest gathering in the world of professionals concerned with the production, care and use of laboratory animals. Scheduled to take place in Denver from October 13–17, this year’s meeting — AALAS’s 70th — seems especially promising, with an exciting slate of workshops, panel sessions and seminars hosted by members of the academic community, research institutions, government organizations and more.
This year’s Spotlight Forum theme — One Health: Connections Between Animal, Human, and Environmental Health — recognizes the connection between the health of people and the health of animals and the environment.

*As an added bonus Consolidated has put together this wonderful list of tips to help first-time attendees make the most out of their experience.
Tips for AALAS National Meeting Attendees
• Keep a copy of the map handy. With hundreds of booths and exhibitors to navigate through, it can be easy to get turned around. Having a map of the exhibit hall at the ready will help keep you on track. You can see the interactive map here.
• Keep a copy of the schedule handy, too. Highlight which workshops and seminars you want to see in advance, so you never miss a session, and be mindful of the time once you arrive. You can get a copy of the preliminary program here.
• Bring plenty of business cards. The AALAS National Meeting is your chance to talk to and learn from thousands of like-minded professionals, so you’ll want to take advantage of every opportunity to network you get.
• Don’t forget your swag bag. From pamphlets to vendor tchotchkes to raffle prizes, there will be ample opportunities to get some really cool free stuff, so make sure you have a bag to carry it all.

To Find out more: https://www.aalas.org/national-meeting

Cleveland, Ohio – Abeona Therapeutics (Nasdaq: ABEO) is a fully-integrated gene and cell therapy company at the forefront of the rapidly-advancing field of genetic medicine. Abeona has standardized on a number of DAI Scientific’s cutting-edge equipment for both their core research laboratories and GMP manufacturing suites.

The Baker Company: SterilGARD Class II Type A2 Biosafety Cabinets

Abeona has selected Baker’s flagship biosafety cabinet for both their everyday cell-culture research applications as well as their GMP manufacturing process, taking advantage of Baker’s available IQ/OQ/PQ validation execution services.

Climet: CI-3100 Trident Real-time Continuous particle counters

Custom-made Climet stainless steel isokinetic probes are installed in each Baker biosafety cabinet, providing real-time particle counts with a sample data memory buffer for up to 10,080 samples.

Hettich: Rotanta 460R Heated/Cooled Centrifuge

German-engineered bench top centrifuge that is able to spin high sample volumes of blood tubes, conical tubes, plates and bottles with a working temperature range of -20º C to +40º C.

LabDesign: Custom mobile/elevating laboratory casework and storage

From initial design to final installation, LabDesign was able to provide creative mobile casework solutions as Abeona transitioned from pre-existing warehouse space to a number of separate research laboratories.

DAI Scientific’s consultative approach helps customers in the bio-pharmaceutical industry hone in on specialized equipment that excels in both R&D laboratories as well as regulated manufacturing facilities.

Autoclave Chamber Material: Stainless Steel vs Nickel-Clad

Current, state-of-the-art laboratory autoclave chambers are manufactured using stainless steel. In the past, autoclave manufacturers constructed sterilizers using nickel-clad, a highly corrosion-resistant material. The industry has shifted due to the rising cost of “cladding” over the last 10-15 years, heading in the direction of primarily 316L stainless steel.

Why should you care?

Because while stainless steel has many excellent properties, some common misconceptions about its versatility can lead to premature corrosion of this material. In short, there is a time and place where nickel-clad chambers are worth the investment.

Stainless Steel Autoclave Chambers

Stainless steel is a versatile material that is used in many industries from food preparation and medical devices to manufacturing equipment and semiconductors. Within the medical and laboratory equipment fields, the most popular metal of choice is “type 316L” stainless steel. This material has gained so much popularity because of its strength, stability, and ease of cleaning.

But although stainless steel is the preferred method of construction, there are many instances where nickel-clad should be considered. As its name implies, stainless steel is “less” prone to staining (and corrosion) than regular steel but unfortunately, it is not stain “proof”. Let’s explore this topic further.

Causes of Corrosion

Corrosion of stainless steel autoclave chambers can occur from a number of different sources ranging from poor water or steam quality to chemical contamination. Here are some common culprits:

  • Chlorides, sulfates, chlorine, hypochlorites, bleach, and acids aggressively attack stainless steel and can cause significant damage to the autoclave chamber and plumbing.
  • Hypochlorites, acids and bleaches are so caustic that they should never be sterilized or used to clean an autoclave. Keep in mind that autoclaves require a combination of heat and moisture to perform proper sterilization. So when high-temperature steam is combined with certain chemicals, the corrosion within the autoclave is accelerated.
  • High-concentration salt solutions such as seawater have a large amount of chlorides and should not be sterilized in stainless steel autoclaves.
  • Tap water with high amounts of chlorine or chlorides should not be used to generate steam for a stainless steel autoclave.

Nickel-Clad Autoclave Chambers

If high-concentration salt solutions (see above) will be sterilized in the autoclave or if the feed-water or tap-water for steam generation has high chloride content, then purchasing a sterilizer with a nickel-clad chamber (versus stainless steel) is recommended. Even though stainless steel has many benefits, nickel-clad is superior to stainless steel in conditions with high chloride concentrations.

The term “nickel-clad” stems from a process called Cladding. In the case of an autoclave chamber, the cladding process involves nickel being bonded to carbon steel to improve its durability and strength. Cladded steel plate is often used in corrosive environments where other materials or coating methods are not suitable.

Best Practices for All Autoclave Chambers

Cleaning

The life of the autoclave will be extended and damage will be greatly minimized if the chamber is cleaned immediately after each use. Look for an autoclave chamber cleaning solution that is specially formulated to remove contaminants on a stainless steel surface – the last thing you want to do is use a cleaning agent that corrodes the autoclave chamber.

Passivation

If solutions with mild chloride content will be sterilized, then “passivation” of the autoclave chamber is recommended. Passivating a stainless steel chamber occurs at the factory and is a process that makes the surface more corrosion resistant by restoring its protective oxide layer. The combination of passivation coupled with diligent cleaning of the chamber will greatly extend the chamber life.

It should be noted that typical LB, agar, and tryptic-soy solutions have a much lower chloride content than high-salt solutions and in turn are safer for stainless steel autoclaves.

 

Here are a couple of pictures from Indiana University and a recent install of nickel clad units and a picture of the old stainless steel unit.

Previous Stainless Steel Unit

New Nickel Clad Unit

Logan, UT, July 27, 2018 – ELITechGroup Biomedical Systems (EBS), known for worldwide transformation and innovation of sweat testing with the Webster Sweat Inducer and Macroduct? Sweat Collection System, endorsed by the U.S. Cystic Fibrosis Foundation and other global CF associations, announces the release of the new FDA-cleared Macroduct® Advanced Sweat Collection System.

Features of the new Macroduct Advanced include a touch screen user interface with built-in step-by-step graphical instructions that further standardize pilocarpine iontophoresis and sweat collection for optimum results. In addition, the revised shape of electrodes and Macroduct collector offer greater convenience when working with neonates and toddlers with small limbs. This system truly is sweat testing at its best while enhancing laboratory workflow in meeting protocols and traceability requirements with flexible options for data management.

“More than 5 million sweat tests have been performed with the existing Macroduct Sweat Collection System. This new Macroduct Advanced proves our commitment to ongoing innovation to improve patient care by developing market-leading diagnostic products. Laboratories that use our products for sweat testing do so with confidence as a result of our superior features that test and troubleshoot for optimal results,” says Bryce McEuen, Vice President, Biomedical Systems.

Sweat testing at its best has arrived. Visit MacroductAdvanced.com

 

The University of Michigan performed extensive research of many brands of Ultra-Low Freezers and the PHCbi (formally Panasonic HealthCare) VIP ECO line was the best option for Energy Savings, Temperature Performance, Reliability, and Sample Storage. This Biorepository has been using the previous VIP freezers with much success and moving to the ECO model was the most natural choice.

 

PHCbi ECO Freezers are available in two upright cabinet sizes (25.7 cu.ft. and 18.6 cu.ft) and in both 115V and 220V electrical models. All VIP ECO freezers have earned ENERGY STAR Certification and include Advanced Frost Control and an EZLatch door design ultimately essential to better energy efficiency in real-world conditions.

D.A.I. Scientific Equipment and PHCbi are proud to be chosen to be the preferred vendor for -80C freezers at this University of Michigan biorepository.

Natural Refrigerant -86°C Ultra-Low Temperature Freezers

The VIP ECO® ultra-low temperature freezer product line includes the prestigious Good Design award-winning -86°C upright model which was also independently rated as the world’s most energy-efficient and best performing ultra-low freezer. Operating on natural refrigerants, all VIP ECO Series freezers are ENERGY STAR® Certified and available in both 115V and 220V models. Our freezers are design, tested and field-proven for safe, reliable storage of biologicals.

High performance and dependability are central to the PHC sustainability equation. Smart compressors and integrated electronics combine with natural refrigerants to lower operating costs without putting reliability and ultra-low temperature performance at risk.

EXPLORE PRODUCT ANIMATIONS

Sustainability Advanced

New natural refrigerants minimize environmental impact without compromising ultra-low temperature performance.

  • Fast pull-down to -80°C demonstrates reserve cooling power
  • Fast recovery after door openings protects stored biological product integrity
  • Narrow interior uniformity, top to bottom, side to side, front to back minimizes uncertainty
  • Patented VIP Series cabinet design slows warm-up during a power outage, saves energy
  • Unique internal heat exchanger increases performance envelope, tolerates high ambient temperatures

The CLARIOstar Plus® is BMG LABTECH’s most flexible microplate reader, equipped with their revolutionary LVF monochromator™ technology. A combination of monochromators, filters, and spectrometer means that it does not compromise on sensitivity or flexibility. As well as its high performance in all detection modes, the CLARIOstar’s versatility also makes it ideal for assay development.

The technical specifications are listed here: https://www.bmglabtech.com/clariostar-plus/

Or check out this great video below!

 

Replacing an autoclave is never an easy task because they are a key component of any facility that cannot be down for an extended period of time. They are also expensive to replace. Besides the cost of buying a new unit, you also have cost to remove the old unit, and possibly have to upgrade the utilities to accept a modern piece of equipment. Despite these costs, one of my customers found it was worth it in a way they never expected!

 

I met with a leading scientific device company in the Chicago area that had a very reliable, yet very old autoclave (over 30 years old), that they knew had to be replaced.  They used indicators to show the unit was properly sterilizing the load but over time the inside chamber was eroding. The challenge was replacing this unit would mean their production would be put on hold. After several meetings with the end-users, their maintenance department, and our local service company, it was decided to keep the old autoclave running and place the new autoclave in a new area. This way the old autoclave could still be used while the new autoclave could be installed and validated. Soon as the validation ended, the new autoclave was put in service and the old autoclave was removed.

 

The customer decided to buy an autoclave from Consolidated with the new X1 controller and on-site validation. The new autoclave was more efficient in the use of utilities (water & electricity), the pre & post vac cycles were more efficient because of a modern vacuum system, and the controller allowed them more flexibility in cycle parameters. The on-site validation proved the new autoclave was so much more efficient it allowed for shorter cycle times compared to the old autoclave. This meant that they could autoclave more product in a day. Along with faster cycle times, they saw a lower scrap rate in what they autoclaved. Below is a portion of an email the customer sent to me…

 

“I think you would be interested in the efficiency gains provided by the new machine.  Our scrap rates have dropped significantly.  On our larger selling product, the rates went from ~8% down to 4%.  The result is actually even better than that as the new scrap rate actually dropped to ~2%.  To mitigate the higher scrap rate, we had incorporated a cap on the forceps to prevent “rusting”.  We decided to remove that cap and the scrap rates only rose to ~4% on the new machine, so this has saved us cost and time. On a smaller selling kit, the rate has dropped from 30% to that same ~4%. Remember we have indicators via both individual pouches and some mixed into the load.  What I’m referring to scrap isn’t failed sterilization, but when the item appears to have been contaminated, aka “rusty”.  That “rust” look would either appear on the metal product, or on the outside of the pouches.  I believe a major contributor to that sort of failure before was the “rusty” dust that aggregated on the inside of the old chamber.”

 

In the end, the cost and time of replacing this autoclave were significant, but in the long run, the faster cycle times and less scrap made up for it.

 

About the Bactron Line

BACTRON chambers are total anaerobic process workstations. Samples can be prepared, cultured, and inspected in the oxygen-free chamber
and standard incubator. Unlike anaerobic jars and bags, which unavoidably expose samples to oxygen, BACTRON units provide complete
anaerobic environments for all your applications.

BACTRON Quality

BACTRON workstations are designed and built to provide years of performance. The chamber is continuously welded stainless steel, and the
front panel is rigid, heavy acrylic for unobstructed vision and chamber integrity.
The gloveless sleeve design forms a comfortable seal around the operators’ arms, permitting bare handed manipulation of plates and
specimens inside the chamber. Since specimens can be kept in the dedicated incubator, the chamber remains at ambient temperature, making
the work area much more comfortable and efficient.

What’s New

With new performance upgrades, BACTRONs have unmatched performance and efficiency. All BACTRONs now have:

BACTRON Applications:

• Clinical Microbiology • Microbiome Studies • Susceptibility Assays • Fermentation and Spoilage Studies • Food Microbiology
• Bio-Energy Research • Environmental Microbiology • Anaerobic Bacteriology

LEARN MORE TODAY