Absorbance and Fluorescence Quantification

DS-11 Series Spectrophotometer / Fluorometer - Technical Note 156

The DeNovix® DS-11 Series Spectrophotometer / Fluorometer enables precise absorbance and fluorescence quantification across a wide dynamic range. The dual mode spectrophotometer is equipped with SmartPath® Technology, which facilitates accurate and reproducible measurements for both cuvette and 1 μL absorbance modes. The proprietary optical core of the fluorescence component utilizes LED excitation sources and highly sensitive photodiodes capable of detecting minute amounts of fluorescence across four wavelength ranges.

The purpose of this document is to describe and compare the complementary methods of absorbance and fluorescence quantification.

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