Importance of Maintaining CO2 Levels One of the greatest challenges scientists face is the reproducibility and accuracy of data. In life science research, this data often rests on the integrity of cell cultures. Cell cultures require proper CO2 control to maintain pH values in the media essential to cell growth. The pH value in the cell culture medium is regulated by the supply of CO2 in the chamber. In order to maintain proper cell health, the incubator should ideally keep pH at a neutral level.