Satisfactory cleaning and decontamination of labware directly impacts the outcome of laboratory research results. Effective laboratory processes must include labware free of soil, grease or other residual impurities that can contaminate active processes or samples, and lead to false outcomes. There are several key factors that determine consistency in the labware washing process, whether manual or automatic. Industry professionals re- fer to these factors as the Sinner’s Circle which includes crucial functions of an effective wash process. This report details the challenges and problems associated with manually washed labware versus labware cleaned in automatic washing systems, and illustrates in context the Sinner’s Circle as it applies to efficacy and repeatability.