Whitepaper
Measurement of red fluorescent dyes for improved multiplexing capabilities and cellular assays on the CLARIOstar® Plus
Fluorescence-based assays conquered the benches of life science labs due to numerous advantages: fluorophores are often non-toxic, they are affordable and can be measured multiple times.
Their intensity is linearly related to their concentration and last but not least the number of assays available is massive. Not only did the use of fluorescence assays per se increase but in particular the use of dyes emitting in the red and infrared range of light has risen. What drives the shift to red dyes?