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Multi-Gas Incubators

Multi-gas incubators use a combination of CO2 and nitrogen gases to simulate in vivo conditions. PHCbi (formerly Panasonic) uses a solid zirconia O2 sensor to control the oxygen levels within the incubator. All models have built-in contamination control features with additional options, which can give your lab an extra level of security.

Multi-gas Incubator Considerations

  • CO2 and O2 control: PHCbi incubators are equipped with dual infrared CO2 and solid-state Zirconia O2 sensors. These sensors enable continuous CO2 calibration and an accurate O2 control range of 1% to 18% and 22% to 80% without periodic calibration.
  • Contamination control: Each of our PHCbi incubators comes with built-in contamination safeguards. All units feature inCu-saFe® interiors that combine the germicidal properties of copper with the corrosion resistance of stainless steel. Additional features include contaminant-destroying UV lights and hydrogen peroxide vapor decontamination options.
  • Event management: Stay notified about deviations from presets and other events with power failure, temperature and gas deviation, supply empty and door open alarms.
  • Gas consumption: Multi-gas incubators go through their supply of nitrogen much more quickly than their CO2 supply. PHCbi incubators come standard with a nitrogen switchover system to provide efficient distribution.
  • Placement and location: To ensure proper ventilation and access to cords and sockets, it’s crucial to choose a location with sufficient surrounding space (at least three inches). The chosen area should also be away from drafts and out of direct sunlight.
  • Size: At D.A.I., the multi-gas incubators we offer are capable of fitting comfortably on a tabletop. With PHCbi MCO-50M models measuring at 1.7 cubic feet and MCO-170MP models measuring at 5.7 cubic feet.
  • Temperature: All of our multi-gas incubator units are equipped with a direct heat and air jacket system that helps maintain accurate temperature levels, ranging from +5°C above ambient to +50°C.

Common Multi-gas Incubator Applications

Multi-gas incubators are designed to create optimal cell culturing environments through the precise regulation of CO2, O2 and temperature. As a result, these incubators are used in a variety of scientific processes that require in vivo oxygen levels, such as:

  • Cancer research
  • Drug testing
  • In vitro fertilization (IVF)
  • Regenerative medicine
  • Stem cell research

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What are multi-gas incubators used for in a lab?
Multi-gas incubators are most frequently used in hypoxia and hyperoxia studies where controlling oxygen levels is needed to support the growth of human and animal cells for research and clinical applications.
How do multi-gas incubators work?
To create the optimal environment for cell growth, these incubators use CO2 and Nitrogen gas. The Nitrogen gas is used to control oxygen levels.
What is the difference between a multi-gas incubator, a microbiological incubator and a CO2 incubator?
While each incubator type shares a similar function — to create optimal environments that support culture growth — they achieve this end in different ways:
  • Multi-gas incubators simulate in vivo conditions using a combination of Nitrogen and CO2 gasses.
  • Microbiological incubators solely control temperature.
  • CO2 incubators use CO2 to control the pH of the cultures stored inside the chamber.
Is it safe to stack multi-gas incubators?
Yes. Our multi-gas incubator models can be safely stacked in two- or three-layer configurations.
How big are multi-gas incubators?
Incubator sizes vary depending on manufacturer and unit type. At D.A.I., our multi-gas incubators come in two sizes: 1.8 cubic feet and 5.7 cubic feet.
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