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Cryogenic Liquid Nitrogen Freezers

Cryogenic storage systems provide a reliable cryopreservation solution to store clinical or research samples at -150°C or colder. These systems include liquid nitrogen autofill freezers, manual fill dewars and cryoshippers (also known as vapor shippers).

We offer autofill liquid nitrogen freezers for applications requiring storage between -135°C and -196°C in either liquid or vapor phase. Microprocessor controls automatically monitor and adjust the liquid levels, providing a secure and reliable long-term storage solution. Stainless steel inventory systems are available for both vial and blood bag storage. Custom systems can be designed for unique applications.

Our CryoMizerTM manual fill dewars combine the ease of sample storage in boxes with the advantage of low liquid nitrogen consumption. The unit allows for storing samples in a liquid or vapor phase.

If you’re in the market for an alternative to liquid nitrogen cryogenic storage freezers, the PHCbi Cryogenic Series -150°C is a great option. This ultra-low temperature mechanical freezer is ideal for cryogenic and long-term storage in laboratories and provides an economical alternative to liquid nitrogen storage.

Common applications for cryogenic storage include the storage and transport of the following: Blood, cord blood, stem cells, DNA, RNA, and other biomedical samples.

Cryogenic Liquid Nitrogen Freezer Considerations


  • Autofill vs. manual: It comes to customer preference. Some people like the fact that LN2 is simple to use and very reliable. Also, if there’s a problem, the repairs aren’t as expensive. For smaller amounts of samples, many people opt for a manual fill. For larger quantities, it is more convenient to use an auto fill LN2 freezer.
  • Temperature: Autofill liquid nitrogen freezers are designed for applications requiring storage between -135°C and -196°C in either liquid or vapor phase.
  • Total vial capacity: Depending on the model, our manual fill units will hold anywhere from 875 to 7,000 vials; our autofill units will hold anywhere from 7,000 to 94,500 vials.
  • Total liquid nitrogen capacity: Autofill units have a capacity of 165 to 300  liters; manual fill units have a capacity of 47 to 175 liters.
  • Cost of cryogenic storage: If you’re looking for an economical alternative, consider the PHC -150°C cryogenic freezer. This mechanical, compressor-based freezer saves you the cost of purchasing liquid nitrogen. This unit, which holds a maximum of 15,000 vials, has a temperature control range of -125°C to -150°C and provides insulation for temperature uniformity for long-term storage. This unit also maximizes storage space with a minimal footprint of 8.2 cubic feet. The typical autofill freezer uses liquid nitrogen as a refrigerant, which means it does not have a compressor to make the samples cold. It’s important to note that each freezer will go though one 160-liter supply tank in a week to 10 days. The cost of this tank is around $100 to $150. This means if you are an autofill user, you will have to buy 3-4 tanks per month, which equates to around $7,000 a year in liquid nitrogen. If you use the PHC -150°C freezer, you do not need to buy the liquid nitrogen because it has compressors to hold temperature of -150 °C. There are a few downsides to a mechanical compressor. It is expensive to repair, and if the unit does fail, it will warm up quickly. That means you need to have a contingency plan in place for your samples.PHC does have an LN2 back-up option, which means you would have a supply tank connected to the freezer. This back-up will allow your samples to stay at critical cryogenic temperatures during a mechanical or power failure. The problem, however, is the LN2 will evaporate at room temperature, meaning it will be completely gone in about 30 days. This means that even if you do not use the supply tank, you still have to replenish the tank.
  • Warranty: All autofill and manual fill models come equipped with a three-year warranty on parts and labor and a five-year warranty on the vacuum.
  • Additional specifications: Depending on the model, our units also feature event and alarm logging, battery backup, enhanced level accuracy with direct read sensors, stainless steel storage racks, a hinged lid, protective boot and lid lock provisions for safety.


My experience with DAI Scientific has been exceptional. I have come to appreciate the reliability of their products, the professionalism of their employees, and the readiness of their responses whether at the quoting, purchasing, delivery or setup stage. I have purchased multiple items from them over the years and their performance and endurance has been outstanding. I frequently recommend DAI to my colleagues and their experience has been as good as mine.
Wayne State University
What is cyro storage?
Cryo storage is designed for the long term storage of samples using mechanical cooling or liquid nitrogen to ensure -150°C or below temperatures.
What does cyrogenic mean?
Cryogenics is a branch of physics that refers to the production and effects of materials at extremely low temperatures. The cryogenic temperature range has been defined as from -150°C (-238°F) to absolute zero (-196 °C or -320°F), the temperature at which molecular motion comes as close as theoretically possible to ceasing completely.
How does an autofill freezer work?
An autofill freezer requires being connected to a liquid nitrogen supply. Storage tanks use vacuum insulation to hold liquid nitrogen. There is a special cryogenic hose that runs from the supply tank to the freezer. The freezer has a microprocessor that controls the liquid levels via sensors and a solenoid valve. Samples can be stored submerged in the LN2 liquid (called liquid phase storage) or have the samples stored above the liquid (called vapor phase storage).
How does a manual fill dewar work?
A dewar is a storage tank that will hold samples and liquid. Similar to an autofill, customers can have samples stored in liquid or vapor phase. Unlike an autofill freezer, there is no supply tank connected to the dewar. The customer has to manually fill each dewar.
What prevents liquid from spilling in a cryoshipper/vaporshipper?
These shippers utilize a highly effective hydro-phobic absorbent that absorbs the LN2 and maintains cryogenic temperatures while eliminating spillage in transit.
How much is a cryogenic freezer?
The price range varies between $2,000 for a small manual fill dewar to $75,000 for our largest auto fill tank. Pricing depends also on the type of inventory racks/system. Custom storage racks are available depending on the type of samples you intend to store.
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